CAMK2A Antikörper (pThr286)

Produktnummer ABIN492420

Der Maus Monoklonal anti-CAMK2A Antikörper (Klon 22B1) (ABIN492420) detektiert spezifisch CAMK2A in WB, IF, IP, EIA und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Ratte.

Kurzübersicht für CAMK2A Antikörper (pThr286) (ABIN492420)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CAMK2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 22B1

Produktdetails anti-CAMK2A Antikörper

Bindungsspezifität: pThr286

Kreuzreaktivität (Details): Species reactivity (tested):Rat, Human.

Produktmerkmale: Synonyms: CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-IIsubunit alpha, Calcium/calmodulin-dependent protein kinase type II subunit alpha

Aufreinigung: Protein G Chromatography.

Immunogen: Synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Immunflourescence: 10 μg/mL. Immunoprecipitaion. ImmunohistochemistryWestern blot: 1 μg/mL, A band of approx. 50 kDa is detected.1 μg/mL of CAMKII was sufficient for detection of 0.2 μg CAMKII by colorimetric immunoblot. (Ref.13)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS pH 7.4 containing 0.09 % Sodium Azide in 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Andere Bezeichnung: CAMK2A

Hintergrund: CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca 2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).Synonyms: CAMKA, CaM kinase II subunit alpha, CaM-kinase II alpha chain, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II subunit alpha, KIAA0968

Gen-ID: 25400

UniProt: P11275

Pathways: WNT Signalweg, Interferon-gamma Pathway, Myometrial Relaxation and Contraction