Acetylated Lysine Antikörper (acetylated, Lys25)

Produktnummer ABIN488677

Der Maus Monoklonal anti-Acetylated Lysine Antikörper wird verwendet zum Nachweis von Acetylated Lysine in Proben von Rind (Kuh) und Avian. Er wurde validiert für WB, ELISA, IHC, IP, IF und ICC.

Kurzübersicht für Acetylated Lysine Antikörper (acetylated, Lys25) (ABIN488677)

Target: Acetylated Lysine

Reaktivität: Rind (Kuh), Avian

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Acetylated Lysine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 7F8

Produktdetails anti-Acetylated Lysine Antikörper

Bindungsspezifität: acetylated, Lys25

Verwendungszweck: Anti-Acetylated lysine (25 μl, clone 7F8)

Spezifität: The antibody detects proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues.

Kreuzreaktivität (Details): Bovine, avian
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Higher plants

Aufreinigung: Protein G purified

Immunogen: acetylated KLH

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: 1 : 100 (IHC), 1 : 1000 (WB)

Kommentare:

1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Total IgG fraction. Protein G purified.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Details zu Acetylated Lysine

Target: Acetylated Lysine

Substanzklasse: Chemical

Hintergrund:

Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).

Additional information: Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/mLantibody detects Proteins containing acetylated lysine residues in ELISA and WBs, Does not detect non-acetylated lysine residues