ATP6V0D2 Antikörper (epsilon subunit)

Produktnummer ABIN488577

Der Ziege Polyklonal anti-ATP6V0D2 Antikörper (ABIN488577) detektiert spezifisch ATP6V0D2 in WB und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Arabidopsis thaliana, Nicotiana tabacum, Tomato, Avena strigosa, Oryza sativa und Alfalfa.

Kurzübersicht für ATP6V0D2 Antikörper (epsilon subunit) (ABIN488577)

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Reaktivität: Arabidopsis thaliana, Nicotiana tabacum, Tomato, Avena strigosa, Oryza sativa, Alfalfa

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ATP6V0D2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-ATP6V0D2 Antikörper

Bindungsspezifität: epsilon subunit

Verwendungszweck: Anti-V-ATPase | Epsilon subunit of tonoplast H+ATPase (affinty purified, goat antibody)

Kreuzreaktivität (Details): Arabidopsis thaliana, Avena strigosa, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Solanum lycopersicum
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Algae,Chlamydomonas reinhardtii,Hordeum vulgare,Malus domestica,Mesembryanthemum sp.,Petunia sp.,Phaseolus sp.,Physcomitrium patens,Pteris vittata (fern),Ricinus communis,Thellungiella sp.,Zea mays,Vitis vinifera Bull frog,Chicken,Bovine,Drosophila melanogaster,Human,Mouse,Rat

Aufreinigung: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E

Anwendungsinformationen

Applikationshinweise: 1: 600 (IF), 1 : 1000-1 : 3000 (WB)

Kommentare:

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Details zu ATP6V0D2

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Andere Bezeichnung: V-ATPase

Hintergrund: Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

Molekulargewicht: 26 | 31 kDa (Arabidopsis thaliana)

Gen-ID: 826716

UniProt: Q39258

Pathways: Transition Metal Ion Homeostasis, Proton Transport