Albumin (ALB) Antikörper

Antigen: Albumin (ALB)

Synonyme: ALB, MGC179277, alb, pro0883, pro0903, pro1341, MGC88928, CSA, DKFZp459F2310, PRO0883, PRO0903, PRO1341, DKFZp779N1935, Alb1, Alb-1, Albza, LOC100136344

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Ratte (Rattus)

Applikation: Immunodiffusion (ID), ELISA, Immunelektrophorese (IEP), Nephelometrie (Neph), Radiale Immunodiffusion (RID)

Produktnummer: ABIN458707

Produktbeschreibung

Produktmerkmale: Precipitating polyclonal rabbit antiserum to rat serum albumin

Weitere Bezeichnung: Serum Albumin

Immunogen: Highly purified albumin isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Beschreibung: The defined antibody specificity is directed to albumin as tested against rat sera. In immuno-electrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified albumin. Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion against several species sera with the following results:

Spezifität: Cow - chicken - dog - goat - guinea pig ++ horse ± human ± mouse + monkey ± rabbit - sheep - swine ±

Anwendungen

Applikationshinweise: As precipitating antiserum to identify or measure rat albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included. Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Konzentration: Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:64 when tested against normal rat serum in agar block immunodiffusion titrati

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum Preservative: No preservative added.

Lagerung: Lyophilised at +4°C (10 years), reconstituted at or below -20°C (3-5 years), reconstituted at +4°C at least 7 days. The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Beschränkungen: Nur für Forschungszwecke einsetzbar