Smooth Muscle Actin Antikörper (N-Term)

Produktnummer ABIN452371

Dieser Maus Monoklonal Antikörper detektiert spezifisch Smooth Muscle Actin in WB, IF, IHC (p) und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus, Huhn, Rind (Kuh) und Pferd.

Kurzübersicht für Smooth Muscle Actin Antikörper (N-Term) (ABIN452371)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reaktivität: Human, Ratte, Maus, Huhn, Rind (Kuh), Pferd

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Smooth Muscle Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: ASM-1

Produktdetails anti-Smooth Muscle Actin Antikörper

Bindungsspezifität: N-Term

Spezifität: ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. Polypeptide reacting: specific for alpha-smooth-muscle isoform of actin (Mr 43,000). Tumors Specificallly Detected: leiomyosarcoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast. Tested Reactivities on Cultured Cell lines: Stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Bovine, Equine, Rat, Mouse and Chicken.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Synthetic N- terminus decapeptide of alpha-smooth-muscleisoform of Actin.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunofluorescence MicroscopyImmunoblotting (Western). Cytological Material. Immunohistochemistry on Frozen and Paraffin-Embedded Tissue. Pretreatment: With paraffin embedded sections, protease pretreatment is required prior toantibody application. Working Dilution: Ready-to-use for Immunohistochemical application. Incubation Time: 1 h at RT, extended with paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluited at 2-8 °CShelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Hintergrund: The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.Synonyms: ACTSA, ACTVS, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

Gen-ID: 58

UniProt: P68133

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development