HSP90AA1 Antikörper

Produktnummer ABIN452367

Der Maus Monoklonal anti-HSP90AA1 Antikörper (Klon D7alpha) (ABIN452367) detektiert spezifisch HSP90AA1 in WB, IP und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Kaninchen, Rind (Kuh), Huhn und Schwein.

Kurzübersicht für HSP90AA1 Antikörper (ABIN452367)

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Reaktivität: Human, Maus, Ratte, Kaninchen, Rind (Kuh), Huhn, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP90AA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: D7alpha

Produktdetails anti-HSP90AA1 Antikörper

Spezifität: This antibody recognizes 90 kDa proteins corresponding to the molecular mass of hsp90. Hsp90alpha specific for human samples. Can isolate complexes of hsp90, Src kinase and cdc37 (1, 2, 3).

Kreuzreaktivität (Details): Species reactivity (tested):Bovine, Chicken, Human, Mouse, Porcine (Pig), Rat, Rabbit.

Produktmerkmale: Synonyms: HSP90A, HSPC1, HSPCA, Heat shock 86 kDa, HSP86, NY-REN-38, Heat shock protein HSP90-alpha

Aufreinigung: Purified

Immunogen: Full length protein purified from chicken brain, clone D7α

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation (1, 2, 3): 5 μg with 20 μL Protein A beads. Immunohistochemistry on frozen sections.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Mouse IgG in PBS buffer, 0.09 % sodium azide and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu HSP90AA1

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Andere Bezeichnung: HSP90AA1 / HSP90 alpha

Hintergrund: Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (4-7). Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90-regulated proteins that have been discovered to date are involved in cell signaling (8-9). The number of proteins now known to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase(6). When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immune-oadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (10).Synonyms: HSP86, HSP90A, HSPC1, HSPCA, Heat shock 86 kDa, Heat shock protein HSP 90-alpha, NY-REN-38

Gen-ID: 3320

UniProt: P07900

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals