His Tag Antikörper (Biotin)

Produktnummer ABIN401733

Der Biotin-konjugierte Kaninchen Polyklonal anti-His Tag Antikörper (ABIN401733) detektiert spezifisch His Tag in WB, IHC (fro) und EIA.

Kurzübersicht für His Tag Antikörper (Biotin) (ABIN401733)

Target: His Tag

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser His Tag Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-His Tag Antikörper (Biotin)

Sequenz: HHHHHH

Produktmerkmale: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Aufreinigung: Affinity Chromatography

Immunogen: 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide

Anwendungsinformationen

Applikationshinweise: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X Hisepitope. This antibody has been tested by ELISA and Western blotting against both the immunizingpeptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for Immunoprecipitation andImmunocytochemistry. Recommended Dilutions:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.1 mL of deionized water (or equivalent). For extended storage, mix with Glycerol to 50 % final

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Prior to and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE!

Details zu His Tag

Target: His Tag

Andere Bezeichnung: 6xHistidine Epitope Tag

Substanzklasse: Tag

Hintergrund: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged proteins biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.