NCOA3 Antikörper (Internal Region, Isoform A)

Produktnummer ABIN401408

Der Kaninchen Polyklonal anti-NCOA3 Antikörper wird verwendet zum Nachweis von NCOA3 in Proben von Human und Affe. Er wurde validiert für WB und EIA.

Kurzübersicht für NCOA3 Antikörper (Internal Region, Isoform A) (ABIN401408)

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCOA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-NCOA3 Antikörper

Bindungsspezifität: Internal Region, Isoform A

Spezifität: This antibody reacts with several isoforms of NCOA3 protein (a, b, d, e and f).

Kreuzreaktivität (Details): Species reactivity (expected):Marcaque, Canine, Bovine, Mouse, Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide corresponding to an internal region of human NCOA3 isoform a protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/175000. Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.24 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu NCOA3

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Andere Bezeichnung: NCOA3

Hintergrund: The NCOA3 (also known as nuclear receptor coactivator 3 isoform a) is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions in a hormone-dependent fashion. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. NCOA3 probably acts via remodeling of chromatin and is involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 is also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. This antibody was raised against variant 1 which is the longer isoform (a). In addition, a polymorphic repeat region is found in the C-terminus of the protein.Synonyms: ACTR, AIB-1, AIB1, Amplified in breast cancer 1 protein, BHLHE42, CBP-interacting protein, Class E basic helix-loop-helix protein 42, NCoA-3, Nuclear receptor coactivator 3, RAC-3, RAC3, Receptor-associated coactivator 3, SRC-3, Steroid receptor coactivator protein 3, TRAM-1, TRAM1, Thyroid hormone receptor activator molecule 1, pCIP

Gen-ID: 8202

NCBI Accession: NP_001167559

UniProt: Q9Y6Q9

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha