PDCD4 Antikörper (pSer457, Ser457)

Produktnummer ABIN401369

Der Kaninchen Polyklonal anti-PDCD4 Antikörper wird verwendet zum Nachweis von PDCD4 in Proben von Human, Maus, Ratte und Xenopus laevis. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für PDCD4 Antikörper (pSer457, Ser457) (ABIN401369)

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reaktivität: Human, Maus, Ratte, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDCD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-PDCD4 Antikörper

Bindungsspezifität: pSer457, Ser457

Spezifität: This antibody is specific for Pdcd4 protein phosphorylated at Ser457.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Xenopus.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids surrounding Ser457 in the human Pdcd4 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/400,000. Western Blot: 1/500 - 1/2,000. Immunohistochemistry: 21.25 - 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.95 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Andere Bezeichnung: PDCD4

Hintergrund: Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

Gen-ID: 27250

NCBI Accession: NP_055271

UniProt: Q53EL6