R-Phycoerythrin Antikörper (Texas Red (TR))

Produktnummer ABIN401163

Der Texas Red (TR)-konjugierte Ziege Polyklonal anti-R-Phycoerythrin Antikörper (ABIN401163) detektiert spezifisch R-Phycoerythrin in FACS und IF.

Kurzübersicht für R-Phycoerythrin Antikörper (Texas Red (TR)) (ABIN401163)

Target: R-Phycoerythrin (Phycoerythrin, R-)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser R-Phycoerythrin Antikörper ist konjugiert mit Texas Red (TR)

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-R-Phycoerythrin Antikörper (Texas Red (TR))

Produktmerkmale: Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 2.5 Moles Texas Red(TM) per mole of Goat IgG

Aufreinigung: Immunoaffinity Chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

Immunogen: Highly purified R-Phycoerythrin from the seaweed gracila

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This product is designed for immunofluorescence microscopy, fluorescence based plateassays (FLISA) and fluorescent western blotting. This product is also suitable for multiplexanalysis, including multicolor imaging, utilizing various commercial platforms. Immunofluorescence: 1/500-1/2500. Flow Cytometry: 1/2000-1/10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL of deionized water (or equivalent) For extended storage, restore with 50 % glycerol

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu R-Phycoerythrin

Target: R-Phycoerythrin (Phycoerythrin, R-)

Andere Bezeichnung: R-Phycoerythrin

Substanzklasse: Chemical

Hintergrund: Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.Synonyms: rpeB

Gen-ID: 2775

UniProt: Q7SIF9