R-Phycoerythrin Antikörper (FITC)
Kurzübersicht für R-Phycoerythrin Antikörper (FITC) (ABIN401159)
Target
Wirt
Klonalität
Konjugat
Applikation
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Produktmerkmale
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Absorption / Emission: 495 nm / 528 nm
Molar Ratio: 3.0 Moles FITC per mole of Goat IgG -
Aufreinigung
- Immunoaffinity Chromatography
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Immunogen
- Highly purified R-Phycoerythrin from the seaweed gracila
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Isotyp
- IgG
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Applikationshinweise
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Immunofluorescence. Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Rekonstitution
- Restore with 1.0 mL of deionized water (or equivalent). For extended storage add glycerol to 50 %
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Konzentration
- 1.1 mg/mL (by UV absorbance at 280 nm)
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Buffer
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (IgG and Protease free)
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeated freezing and thawing.
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- R-Phycoerythrin (Phycoerythrin, R-)
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Andere Bezeichnung
- R-Phycoerythrin
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Substanzklasse
- Chemical
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Hintergrund
- Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.Synonyms: rpeB
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Gen-ID
- 2775
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UniProt
- Q7SIF9
Target
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