GM-CSF Antikörper
| Antigen |
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| Synonyme |
GMCSF, GM-CSF |
| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Human (111), Maus (80), Ratte (Rattus) (31), Rind (Kuh) (20), Hund (17), Huhn (16), Schwein (16), Rhesusaffen (11), Schaf (3), Affe (1), Kaninchen (1)
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Applikation
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 Alternativen Enzyme Immunoassay (EIA), Neutralisierung (Neut), Western Blot (WB)
Western Blot (WB) (98), Enzyme Immunoassay (EIA) (71), Durchflusszytometrie (FACS) (52), ELISA (44), Immunfluoreszenz (IF) (40), Immunpräzipitation (IP) (18), Intrazelluläre Durchflusszytometrie (ICFC) (15), Neutralisierung (Neut) (15), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (14), ELISA (Fangantikörper) (9), ELISA (Detektionsantikörper) (9), ELISPOT (9), Funktionale Studien (Func) (9), Immunhistochemie (Paraffinschnitte) (IHC (p)) (8), Immunhistochemie (IHC) (7), ELISPOT (Fangantikörper) (4), ELISPOT (Detektionsantikörper) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Blockierende Antikörper (Inhibition) (3), Dot Blot (Dot) (3), Radioimmunoassay (RIA) (3), Immunoelectron Microscopy (IEM) (2), Immunoassay (IA) (1)
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| Produktnummer |
ABIN401109 |
| Menge |
0.1 mg (1.0 mg/ml (by UV absorbance at 280 ...) (Varianten) |
| Preis |
310,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulatingfactor, Colony stimulating factor 2
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Swiss-Prot
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P01587
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Immunogen
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This purified antibody was prepared from whole rabbit serum produced by repeatedimmunizations with full length recombinant mouse GM-CSF protein.
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Kreuzreaktivität
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Maus
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Isotyp
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IgG (Passende Sekundärantikörper)
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Beschreibung
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Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF,Colony-stimulating factor, CSF, sargramostim and molgramostin) is produced in responseto a number of inflammatory mediators by mesenchymal cells present in the hemopoieticenvironment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is ableto stimulate the production of neutrophilic granulocytes, macrophages, and mixedgranulocyte-macrophage colonies from bone marrow cells and can stimulate the formationof eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate somefunctional activities in mature granulocytes and macrophages. GM-CSF receptors showsignificant homologies with other receptors for hematopoietic growth factors, includingIL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.
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Spezifität
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This antibody reacts to Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF). Species: Mouse. Others not tested.
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Applikationshinweise
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ELISA: 1/20000 - 1/100000. Western Blot: 1/500 - 1/2000. Neutralization: 1/400. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Konzentration
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1.0 mg/ml (by UV absorbance at 280 nm)
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Reinigung
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Purified
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Buffer
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).
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Lagerung
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Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one monthor (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Forschungsgebiet
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Immunologie, Angeborene Immunität, Zytokine
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "GM-CSF", Typ "Antikörper" finden
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Wirte
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Kaninchen (80), Ratte (72), Maus (48), Ziege (2), Huhn (1), Schaf (1)
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Reaktivitäten
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Human (111), Maus (80), Ratte (Rattus) (31), Rind (Kuh) (20), Hund (17), Huhn (16), Schwein (16), Rhesusaffen (11), Schaf (3), Affe (1), Kaninchen (1)
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Applikationen
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Western Blot (WB) (98), Enzyme Immunoassay (EIA) (71), Durchflusszytometrie (FACS) (52), ELISA (44), Immunfluoreszenz (IF) (40), Immunpräzipitation (IP) (18), Intrazelluläre Durchflusszytometrie (ICFC) (15), Neutralisierung (Neut) (15), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (14), ELISA (Fangantikörper) (9), ELISA (Detektionsantikörper) (9), ELISPOT (9), Funktionale Studien (Func) (9), Immunhistochemie (Paraffinschnitte) (IHC (p)) (8), Immunhistochemie (IHC) (7), ELISPOT (Fangantikörper) (4), ELISPOT (Detektionsantikörper) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Blockierende Antikörper (Inhibition) (3), Dot Blot (Dot) (3), Radioimmunoassay (RIA) (3), Immunoelectron Microscopy (IEM) (2), Immunoassay (IA) (1)
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Konjugate
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Biotin (23), PE (10), FITC (7), Alexa Fluor 647 (3), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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