CD97 Antikörper (Extracellular Domain, N-Term)

Produktnummer ABIN401030

Der Kaninchen Polyklonal anti-CD97 Antikörper wird verwendet zum Nachweis von CD97 in Proben von Maus. Er wurde validiert für WB, IF und EIA.

Kurzübersicht für CD97 Antikörper (Extracellular Domain, N-Term) (ABIN401030)

Target: CD97

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD97 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-CD97 Antikörper

Bindungsspezifität: AA 1-512, Extracellular Domain, N-Term

Spezifität: This antibody is directed against the CD97 protein. A BLAST analysis was used to suggest cross reactivity with CD97 proteins from Mouse (100 %) and Rat (74 %). Only 49 % homology is noted for the Human homologue. Reactivity against CD97 from other sources is not known.

Kreuzreaktivität (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Mouse, Human, Chimpanzee.

Aufreinigung: Affinity chromatography on Protein A

Immunogen: Recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: 1/1000 - 1/5000. Western Blot: 1/1000 - 1/5000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.1 mL deionized water (or equivalent).

Konzentration: 5.0 mg/mL (UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M NaCl, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu CD97

Target: CD97

Andere Bezeichnung: CD97

Hintergrund: CD97 is a membrane antigen that is either constitutively expressed or induced by activation on cells of the immune system including hematopoietic cells. Particularly high levels of expression are also seen in the lung and the thymus gland. In the spleen, expression is detected on most myeloid cells and variable portions of T cells, B cells and NK cells. In humans, CD97 expression is upregulated during lymphocyte activation on various epithelial cancer cells including colon, thyroid, stomach, and esophagus. One high affinity ligand that has been identified for CD97 is the integrin alpha 5 beta 1. CD97 engagement of endothelial alpha 5 beta 1 results in increased angiogenesis. Signals generated through CD97 are thought to result in Rho activation and the regulation of motility. Antibodies against CD97 are suitable for exploring various mouse genetic models of cancer, angiogenesis, and inflammation. CD97 can be proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. Multiple isoforms have been identified for this protein.Synonyms: Leukocyte antigen CD97

Gen-ID: 26364

NCBI Accession: NP_001156501

UniProt: Q9Z0M6