NEK9 Antikörper (C-Term)

Produktnummer ABIN392654

Der Kaninchen Polyklonal anti-NEK9 Antikörper wird verwendet zum Nachweis von NEK9 in Proben von Human und Maus. Er wurde validiert für WB und IHC (p).

Kurzübersicht für NEK9 Antikörper (C-Term) (ABIN392654)

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEK9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB2989

Produktdetails anti-NEK9 Antikörper

Bindungsspezifität: AA 846-877, C-Term

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This NEK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 846-877 amino acids from the C-terminal region of human NEK9.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Details zu NEK9

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Andere Bezeichnung: NEK9

Hintergrund: NEK9, a member of the NIMA subfamily of Ser/Thr protein kinases, is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. It phosphorylates different histones (serine and threonine residues on H3), myelin basic protein, beta-casein (serine residues), and BICD2. NEK9 is activated during mitosis by intramolecular autophosphorylation. Activity and autophosphorylation is activated by manganese >> magnesium ions, and is sensitive to increasing concentration of detergents. This protein is not cell-cycle regulated but activity is higher in G0-arrested cells. NEK is part of a homodimer that binds to Ran GTPase, and exhibits a greater affinity for Ran-GDP over Ran-GTP. Interaction is also noted with NEK6 and NEK7 family members. This cytoplasmic protein is most abundant in heart, liver, kidney and testis, and is also expressed in smooth muscle cells and fibroblasts. Expression varies mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.

Molekulargewicht: 107168

Gen-ID: 91754

NCBI Accession: NP_149107

UniProt: Q8TD19

Pathways: SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion