PI4K2A Antikörper (N-Term)

Produktnummer ABIN392587

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PI4K2A in WB, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PI4K2A Antikörper (N-Term) (ABIN392587)

Target: PI4K2A (Phosphatidylinositol 4-Kinase Type 2 alpha (PI4K2A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PI4K2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB1679

Produktdetails anti-PI4K2A Antikörper

Bindungsspezifität: AA 1-30, N-Term

Aufreinigung: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This PI4K2A (PI4K II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PI4K2A (PI4K II).

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:10~50. WB: 1:8000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Details zu PI4K2A

Target: PI4K2A (Phosphatidylinositol 4-Kinase Type 2 alpha (PI4K2A))

Andere Bezeichnung: PI4K2A (PI4K II)

Hintergrund: Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs. PI4K II is activated by detergent and inhibited by adenosine. Overexpression of PI4KII in COS-7 cells increases synthesis of PtdIns4P. Some cells overexpressing PI4KII have scattered or no perinuclear Golgi. Knockdown of PI4KII by RNA interference (RNAi) does not disrupt the Golgi, and some cells show expanded Golgi. RNAi reduces the Golgi level of PtdIns4P and blocks the association between AP1 and the trans-Golgi network. PI4KII RNAi had little effect on intra-Golgi trafficking, but it inhibited export to plasma membrane export by 35 % . It has been proposed that PI4KII generates PtdIns4P-rich domains within the Golgi that specify docking of the AP1 coat machinery.

Molekulargewicht: 54022

Gen-ID: 55361

NCBI Accession: NP_060895

UniProt: Q9BTU6

Pathways: Inositol Metabolic Process, Synaptic Membrane