phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE) (Center) Antikörper

Antigen: phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE)

Synonyme: p235, Pip5k, Pip5k3, Pipk5k3, 5230400C17Rik, CFD, FAB1, PIP5K, PIP5K3, KIAA0981, MGC40423, Pikfyve, pip5k3, wu:fo93c03, PIKFYVE

Epitope: Center

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN391254

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: PIP5K3 (Center)

Gen-ID: 200576

Swiss-Prot: Q9Y2I7

Immunogen: This PIP5K3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~48 amino acids from the center region of human PIP5K3.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: FYVE finger-containing phosphoinositide kinase, 1-phosphatidylinositol-4-phosphate 5-kinase, Phosphatidylinositol-3-phosphate 5-kinase type III, PIP5K, PtdIns(4)P-5-kinase, PIKfyve, p235, PIP5K3, KIAA0981, PIKFYVE,

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human PIP5K3.

Molekulargewicht: 237108 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Immunohistochemistry~~1:50~100 Western blotting ~~ 1:50~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Phospho-Antikörper, Proteinmodifikationen, Zellstruktur, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of PIP5K3 (arrow) using rabbit polyclonal PIP5K3 Antibody (Center) (ABIN391254). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K3 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publikationen

Publikationen: Alesutan, Ureche, Laufer et al.: "Regulation of the glutamate transporter EAAT4 by PIKfyve." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 25, Issue 2-3, pp. 187-94, 2010 (PubMed).