Eukaryotic Translation Initiation Factor 2 alpha Kinase 4 (EIF2AK4) (N-Term G11) Antikörper

Antigen: Eukaryotic Translation Initiation Factor 2 alpha Kinase 4 (EIF2AK4)

Synonyme: GCN2, KIAA1338, EIF2AK4

Epitope: N-Term G11

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN391197

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: GCN2 (N-term G11)

Gen-ID: 440275

Swiss-Prot: Q9P2K8

Immunogen: This GCN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region with glycine at position 11 of human GCN2.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein, EIF2AK4, GCN2, KIAA1338,

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region with glycine at position 11 of human GCN2.

Molekulargewicht: 186838 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Western Blot ~~ 1:100~500 Immunohistochemistry~~1:50~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Translationsfaktoren, Phospho-Antikörper, Zelluläre Signaltransduktion, Proteinmodifikationen, Transkriptionsfaktoren, Signaltransduktion, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of GCN2 (arrow) using GCN2 Antibody (N-term G11) (ABIN391197). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GCN2 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publikationen

Publikationen: Lu, Luaszluo, Miao et al.: "The role of nitric-oxide synthase in the regulation of UVB light-induced phosphorylation of the alpha subunit of eukaryotic initiation factor 2." in: The Journal of biological chemistry, Vol. 284, Issue 36, pp. 24281-8, 2009 (PubMed).