MKNK2 Antikörper (N-Term)

Produktnummer ABIN391072

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch MKNK2 in WB. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für MKNK2 Antikörper (N-Term) (ABIN391072)

Target: MKNK2 (MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MKNK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: RB5445

Produktdetails anti-MKNK2 Antikörper

Bindungsspezifität: AA 70-100, N-Term

Homologie: M, Rat

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This MNK2 (MKNK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-100 amino acids from the N-terminal region of human MNK2 (MKNK2).

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Details zu MKNK2

Target: MKNK2 (MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2))

Andere Bezeichnung: MNK2 (MKNK2)

Hintergrund: MAP kinase-interacting kinase 1 (Mnk1) and Mnk2, members of the Ser/Thr protein kinase family, bind tightly to the growth factor-regulated MAP kinases, Erk1 and Erk2. Erk and p38 phosphorylate MNK1 and Mnk2, which stimulates their in vitro kinase activity toward a substrate, eukaryotic initiation factor-4E (eIF-4E). Overexpression of Mnk2 results in increased phosphorylation of endogenous eIF-4E, showing that it can act as an eIF-4E kinase in vivo. Mnk2 may play a role in the response to environmental stress and cytokines. This ubiquitiously expressed protein appears to regulate transcription by phosphorylating eIF-4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Expression of active mutants of MNK1 and MNK2 in 293 cells diminishes cap-dependent translation relative to cap-independent translation in a transient reporter assay. Human Mnk2 is homologous to murine Mnk2 (approximately 94 % identical) and human Mnk1 (71 % identical). In vitro phosphorylation studies show that Mnk2 is a significantly better substrate than Mnk1 for extracellular signal-regulated kinase 2 (Erk2), p38MAPKalpha, and p38MAPKbeta. Mnk2 has also been shown to interact with the C-terminal regions of eIF-4G1 and eIF-4G2.

Molekulargewicht: 51875

Gen-ID: 2872

NCBI Accession: NP_060042, NP_951009

UniProt: Q9HBH9

Pathways: MAPK Signalweg