Pyruvate Kinase, Muscle (PKM2) (C-Term) Antikörper

Antigen: Pyruvate Kinase, Muscle (PKM2)

Synonyme: PK3, PKM, TCB, OIP3, CTHBP, THBP1, MGC3932, Pk3, Pk-2, Pk-3, AA414905, AL024370, AL024424, PKM12, Pyk, DmelCG7070, CG7070, pkm2, cb1029, fj33f03, wu:fj33f03, MGC53388, PKM2, PYK3b, PYK3c, PK-S

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus), Primate

Applikation: Western Blot (WB), Immunhistochemie (IHC), Immunfluoreszenz (IF), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN391053

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: Pyruvate Kinase (PKM2) (C-term)

Gen-ID: 5315

Swiss-Prot: P14786

Immunogen: This PKM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 483~513 amino acids from the C-term region of human PKM2.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Pyruvate kinase, isozymes M1/M2, Pyruvate kinase muscle isozyme, Cytosolic thyroid hormone-binding protein, CTHBP, THBP1, PKM

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-term region of human PKM2.

Molekulargewicht: 57805 DA

Anwendungen

Applikationshinweise: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~1:100 Immunofluorescence~~1:10~50 Flow cytometry

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Phospho-Antikörper, Stammzellen, Zelluläre Signaltransduktion, Proteinmodifikationen, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of anti-h PKM2-N491 Pab (ABIN391053) in Ramos cell line lysates (35ug/lane). hPKM2-N491(arrow) was detected using the purified Pab (1:60dilution).

Pyruvate Kinase (PKM2) Antibody (C-term) (ABIN391053) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

(Top) Negative control of hela cells without Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).Alexa-Fluor-546 emits orange fluorescence. (Bottom) Immunofluorescence analysis of Pyruvate Kinase (PKM2) Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).