Gremlin (C-Term) Antikörper

Antigen: Gremlin

Synonyme: ld, Drm, Cktsf1b1, DRM, PIG2, DAND2, IHG-2, GREMLIN, CKTSF1B1, MGC126660, drm, GREM1, grem1, cktsf1b1, MGC136702, zgc:136702, pig2, dand2, ihg-2, gremlin

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN390078

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: GREMLIN (C-term)

Gen-ID: 23892

Swiss-Prot: O70326

Immunogen: This GREMLIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154~184 amino acids from the C-terminus of human Gremlin.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Cysteine KNOT superfamily 1, BMP antagonist 1

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminus of human Gremlin.

Molekulargewicht: 20697 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry ~~ 1:50~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Neurologie, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of anti-Gremlin Pab (ABIN390078) in CEM cell line lysates (35ug/lane). Gremlin (arrow) was detected using the purified Pab.

Western blot analysis of anti-Gremlin Pab (ABIN390078) in mouse liver cell lysate. Gremlin (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of primary antibody was 1:50. Detection method was ABC system or Vectastaint Elite VECTOR. Data and protocol courtesy of Dr. Maria E. Burgos of Universidad Austral de Chile.

Publikationen

Publikationen: Jara, Chacuon, Burgos et al.: "Expression of gremlin, a bone morphogenetic protein antagonist,is associated with vascular calcification in uraemia." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 24, Issue 4, pp. 1121-9, 2009 (PubMed).

Cooney, Baker, Brain et al.: "NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation." in: Nature medicine, Vol. 16, Issue 1, pp. 90-7, 2010 (PubMed).