Beclin 1, Autophagy Related (BECN1) Antikörper

Antigen: Beclin 1, Autophagy Related (BECN1)

Synonyme: ATG6, VPS30, beclin1, MGC6843, 4921513J16Rik, 5430417M23Rik, MGC73097, zgc:73097, BECN1, becn1, MGC80672, MGC126978, atg6, vps30, Beclin-1, DKFZp459C0719

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), Durchflusszytometrie (FACS), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN388546

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: Beclin 1

Gen-ID: 8678

Swiss-Prot: Q14457

Immunogen: This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217~247 amino acids surrounding amino acid E225 of human BECN1.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: BECN1, ATG6, VPS30, beclin1, ATG6 autophagy related 6 homolog, beclin 1 (coiled-coil, moesin-like BCL2 interacting protein), beclin 1 (coiled-coil, moesin-like BCL2-interacting protein), beclin-1, coiled-coil myosin-like BCL2-interacting protein

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide surrounding amino acid E225 of human BECN1.

Molekulargewicht: 51896 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Flow cytometric~~1:10~50

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Apoptose/Nekrose, Zelluläre Signaltransduktion, Zellstruktur, Autophagie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

The anti-BECN1 Pab (ABIN388546) is used in Western blot to detect BECN1 in, from left to right, A2058, Hela, mouse brain, Y79, and HL60 tissue lysates. BECN1(arrow) was detected using the purified Pab.

Western blot analysis of anti-hBECN1-E225 Pab (ABIN388546) in 293 cell line lysates transiently transfected with the BECN1 gene (2ug/lane). hBECN1-E225(arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Autophagy BECN1 (APG6) antibody (E225) (ABIN388546), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.