Cleaved LC3B Antikörper

Antigen: Cleaved LC3B

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunfluoreszenz (IF), Immunzytochemie (ICC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN388484

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Gen-ID: 81631

Swiss-Prot: Q9GZQ8

Immunogen: This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96~125 amino acids from the C-terminal region of human cleaved-LC3 (APG8b).

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: MAP1LC3B, LC3B, MAP1A/1BLC3, MAP1LC3B

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the C-terminal cleavage site of human cleaved-LC3 (APG8b).

Molekulargewicht: 14557 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Immunofluorescence ~~ 1:10~50 Immunocytochemistry ~~ 1:50~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Forschungsgebiet: Zelluläre Signaltransduktion, Zellstruktur, Autophagie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Time course study of mouse leukaemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. Cleaved-LC3 (APG8b) antibody (ABIN388484) detected punctuate staining indicative of autophagic vacuole or phagosome structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.

SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Treatment with ABIN388484 antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.

Publikationen

Publikationen: English, Chemali, Duron et al.: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." in: Nature immunology, Vol. 10, Issue 5, pp. 480-7, 2009 (PubMed).