HtrA serine Peptidase 1 (HTRA1) (C-Term) Antikörper

Antigen: HtrA serine Peptidase 1 (HTRA1)

Synonyme: L56, HtrA, ARMD7, ORF480, PRSS11, HTRA, Prss11, RSPP11, AI429470, MGC93339, htra1, zgc:92029, Xhtra1, zgc:172061, l56, htra, prss11, MGC145723

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN388128

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: HtrA1 (C-term)

Gen-ID: 5654

Swiss-Prot: Q92743

Immunogen: This HtrA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389~419 amino acids from the C-terminal region of human HtrA1.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Serine protease HTRA1, L56

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human HtrA1.

Molekulargewicht: 51286 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Forschungsgebiet: Apoptose/Nekrose, Krebs, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

HtrA1-K396 (ABIN388128) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the HtrA1 antibody detected the HtrA1 protein (arrow).

Publikationen

Publikationen: Chamberland, Wang, Jones et al.: "Identification of a novel HtrA1-susceptible cleavage site in human aggrecan: evidence for the involvement of HtrA1 in aggrecan proteolysis in vivo." in: The Journal of biological chemistry, Vol. 284, Issue 40, pp. 27352-9, 2009 (PubMed).