SMT3 Suppressor of Mif Two 3 Homolog 2/3 (SUMO2/3) (C-Term) Antikörper

Antigen: SMT3 Suppressor of Mif Two 3 Homolog 2/3 (SUMO2/3)

Synonyme: HSMT3, SMT3B, SMT3H2, MGC117191, zgc:92241, SUMO2

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (IHC), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN388034

Produktbeschreibung

Produktmerkmale: Purified Rabbit Polyclonal Antibody (Pab)

Weitere Bezeichnung: SUMO2/3 (C-term)

Gen-ID: 6612

Swiss-Prot: P55854

Immunogen: This SUMO2/3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58~88 amino acids within C-terminal region of human SUMO2/3.

Isotyp: Ig  (Passende Sekundärantikörper)

Beschreibung: Other names: Small ubiquitin-like modifier, Ubiquitin-like protein SMT3B, Sentrin 2, HSMT3

Spezifität: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected within C-terminal region of human SUMO2/3.

Molekulargewicht: 11637 DA

Anwendungen

Applikationshinweise: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Forschungsgebiet: Apoptose/Nekrose, Translationsfaktoren, Proteinmodifikationen, Transkriptionsfaktoren, Signaltransduktion, Zellstruktur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

The anti-SUMO2/3 C-term Pab (ABIN388034) is used in Western blot to detect SUMO2/3 in HeLa cell lysate.

COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels: cell lysates probed by western blot (WB) with an anti-ERM antibody. Center panels: cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB with ABIN388027 SUMO-1 antibody. Bottom panels: cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with ABIN388034 SUMO-2/3 antibody. (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.