GFP Tag Antikörper

Antigen: GFP Tag

Klonalität: Monoklonal (168AT1211)

Wirt: Maus

Applikation: Western Blot (WB), Immunfluoreszenz (IF), ELISA

Zertifikate: ISO 9001:2008

Produktnummer: ABIN387748

Produktbeschreibung

Produktmerkmale: Purified Mouse Monoclonal Antibody (Mab)

Immunogen: Purified His-tagged GFP protein was used to produced this monoclonal antibody.

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 168AT1211

Beschreibung: Other names: Green Fluorescent Protein

Spezifität: Purified His-tagged GFP protein was used to produced this monoclonal antibody.

Molekulargewicht: DA

Anwendungen

Applikationshinweise: ELISA~~1:3,000 Western blotting~~1:2,000 Immunohistochemistry~~1:50~100

Konzentration: 0.25 mg/ml

Reinigung: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified by protein G and Ni-NTA nickel affinity chromatography, followed by dialysis against PBS.

Lagerung: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Forschungsgebiet: Tags/Labels

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

(Top)Western blot analysis of anti-GFP Mab (Cat. #ABIN387748) using purified GFP, YFP and BFP proteins expressed in bacteria: Both GFP (Lanes 1-3) and YFP (Lanes 4-6) but not BFP (data not shown) were detected using the purified Mab. (Bottom)Western blot analysis of anti-GFP Tag Antibody (SG071130A) in GFP recombinant protein. GFP recombinant protein (arrow) was detected using the purified Mab. Immuno-fluorescence for GFP Tag Antibody (Cat # ABIN387748). Antibody target is a YFP tagged protein (the inner membrane complex) of Toxoplasma gondii, which localizes around the exterior of the parasite. Brighter parasites represent actively dividing cells and darker ones are not actively dividing. Secondary goat-anti-mouse and goat-anti-rabbit were conjugated with Alexa546 (Molecular Probes)at 1:200 dilution. Phase contrast enhancement applied. Fluorescent lamp exposure times as follows: RFP Channel, 0.075 sec, YFP Channel, 1.000 sec, DAPI Channel, 0.020 sec, Phase Channel: 0.300 sec. Microscope: Axiovert 200M inverted microscope (Zeiss) equipped with a PRIOR stage and a NEOFLUAR 100x/1.3 objective lens. Images were collected with a Hamamatsu C4742-95 CCD camera. Chroma Filtersets: TRITC, HQ545/30x – Q570LP – HQ620/60m, DAPI, D350/50x – E420LP – 400DCLP, YFP, Q151LP – HQ500/20x – HQ535/30m. Data courtesy of Seth Robertson, Boston College.

anti-GFP Tag antibody (Image 2)

Publikationen

Publikationen: Kapoor, Bartoszewski, Qadri et al.: "Knockdown of ASIC1 and epithelial sodium channel subunits inhibits glioblastoma whole cell current and cell migration." in: The Journal of biological chemistry, Vol. 284, Issue 36, pp. 24526-41, 2009 (PubMed).

Maier, Ogihara, Trace et al.: "The unique hypusine modification of eIF5A promotes islet beta cell inflammation and dysfunction in mice." in: The Journal of clinical investigation, 2010 (PubMed).