DYKDDDDK Antikörper

Antigen: DYKDDDDK

Klonalität: Monoklonal (5A8E5)

Wirt: Maus

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Zertifikate: ISO 9001:2008

Produktnummer: ABIN387700

Produktbeschreibung

Immunogen: A synthetic peptide (DYKDDDDK) coupled to KLH

Format: Purified

Isotyp: IgG2b, kappa chain  (Passende Sekundärantikörper)

Klon: 5A8E5

Beschreibung: The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.

Spezifität: The antibody recognizes C-terminal, N-terminal and internal taggedfusion proteins.

Anwendungen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/ml Western blot: 0.1-1.0 ug/mlImmunoprecipitation: 1 ug/ml Other applications: user-optimized

Konzentration: 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Reinigung: Protein A affinity column

Lagerung: Mouse Anti-DYKDDDDK-tag Monoclonal Antibody should be stored lyophilized until use. It is stable in lyophilized form for at least two years if stored at -20°C. The reconstituted antibody can be stored for up to three months at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Forschungsgebiet: Tags/Labels

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Loading:Lane 1.N-terminal DYKDDDDK-fusion protein (400 ng)Lane 2.C-terminal DYKDDDDK-fusion protein (200 ng)Lane 3. Internal DYKDDDDK-fusion protein (Antibodies-Online. M0101, 200 ng)Primary antibody:A .1 µg/ml Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Antibodies-Online, Cat. No. ABIN387700, Lot No. A213902)B. 1 µg/ml Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (from other company)Secondary antibody: IRDye800 Conjugated affinity Purified Goat Anti-Mouse IgG (ROCKLAND, 1:10,000,610-132-121)

Loading:Lane 1-3 Multiple Tag (Purified) (Antibodies-Online, Cat.No.M0101, 400 ng, 80 ng,16 ng)Lane 4. 293 cell lysate 10 µlPrimary antibody:A. 1 µg/ml Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Antibodies-Online, Cat. No. ABIN387700, Lot No. A213902)B. 1 µg/ml Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (other company)Secondary antibody:IRDye800 Conjugated affinity Purified Goat Anti-Mouse IgG (ROCKLAND, 1:10,000,610-132-121)

Lane1: 1ul E-coli lysate with DYKDDDDK-tag protein(MW:57kDa) and 2ug Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Antibodies-Online, ABIN387700) for IPLane2: 1ul E-coli lysate without DYKDDDDK-tag protein (MW:57kDa) and 2ug Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Antibodies-Online, ABIN387700) for IPLane3: purified DYKDDDDK-tag protein