Clusterin Antikörper (C-Term)

Produktnummer ABIN375042

Zitiert in 1 Publikation. Der Ziege Polyklonal anti-Clusterin Antikörper (ABIN375042) detektiert spezifisch Clusterin in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte und Maus.

Kurzübersicht für Clusterin Antikörper (C-Term) (ABIN375042)

Target: Clusterin (CLU)

Reaktivität: Ratte, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Clusterin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Clusterin Antikörper

Bindungsspezifität: C-Term

Sequenz: C-EKALQEYRRK SRAE

Spezifität: This antibody recognizes Clusterin / ApoJ (C-term).

Kreuzreaktivität (Details): Species reactivity (tested):Mouse, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_038520.1. Genename: Clu

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/8000. Western Blot: 0.3-1 μg/mL. Approx.37 kDa band observed in Mouse Brain and Testis lysatesand in Rat Brain and Ovary lysates (calculated MW of 51.7 kDa according to Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-Clusterin Antikörper (ABIN375042)

Charnay, Imhof, Vallet, Hakkoum, Lathuiliere, Poku, Aronow, Kovari, Bouras, Giannakopoulos: "Clusterin expression during fetal and postnatal CNS development in mouse." in: Neuroscience, Vol. 155, Issue 3, pp. 714-24, (2008) (PubMed).

Details zu Clusterin

Target: Clusterin (CLU)

Andere Bezeichnung: Apolipoprotein J (Apo J)

Hintergrund: At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein J is the major secreted product of Sertoli cells and is thought to play a critical role in spermatogenesis. The protein was shown to be a normal constituent of human blood and is also thought to be a control mechanism of the complement cascade. It prevents the binding of a C5b-C7 complex to the membrane of the target cell and in this way inhibits complement-mediated cytolysis. Apolipoprotein J is overexpressed in human prostate and breast cancers and in squamous cell carcinomas. Suppression of Apolipoprotein J renders these cells sensitive to chemotherapeutic drug-mediated apoptosis. It has been proposed that elevated levels of Apolipoprotein J may promote oncogenic transformation and tumor progression by interfering with BAX proapoptotic activities.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2

Gen-ID: 12759, 10090

UniProt: Q06890

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling