KCNJ11 Antikörper (Internal Region, N-Term)

Produktnummer ABIN374930

Der Ziege Polyklonal anti-KCNJ11 Antikörper wird verwendet zum Nachweis von KCNJ11 in Proben von Human, Maus, Ratte und Hund. Er wurde validiert für WB und IHC (p).

Kurzübersicht für KCNJ11 Antikörper (Internal Region, N-Term) (ABIN374930)

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KCNJ11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-KCNJ11 Antikörper

Bindungsspezifität: Internal Region, N-Term

Sequenz: ERRARFVSKK GNC

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat and Canine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region (near the N Terminus) of the protein sequence according to NP_034732.1. Genename: Kcnj11

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.01-0.03 μg/mL. Approx 45 kDa band observed in Human Skeletal Musclelysates (calculated MW of 43.5 kDa according to NP_034732.1). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Pancreas shows variablestaining across the islet of Langerhans.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu KCNJ11

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Andere Bezeichnung: KCNJ11

Hintergrund: ATP-sensitive potassium (K(ATP)) channels are found in endocrine cells, neurons and both smooth and striated muscle, where they play an important role in controlling insulin secretion and vascular tone, and protect neurons under metabolic stress. Kir6.2 is a member of the inward rectifier potassium channel family, which is characterised by a greater tendency to allow potassium flow into the cell rather than out of it. It associates with the sulphonylurea receptor SUR1/ABCC8 to form a subfamily of K(ATP) channels that, when mutated or misregulated, are associated with forms of hyperinsulinemic hypoglycemia, neonatal diabetes, or pre-disposition to type 2 diabetes mellitus.Synonyms: ATP-sensitive inward rectifier potassium channel 11, IKATP, Inward rectifier K+ channel Kir6.2, KCNJ11, Potassium channel, inwardly rectifying subfamily J member 11

Gen-ID: 9606

UniProt: Q14654

Pathways: Negative Regulation of Hormone Secretion