AOC3 Antikörper (C-Term, Internal Region)

Produktnummer ABIN374864

Der Ziege Polyklonal anti-AOC3 Antikörper wird verwendet zum Nachweis von AOC3 in Proben von Human, Maus und Ratte. Er wurde validiert für IHC (p).

Kurzübersicht für AOC3 Antikörper (C-Term, Internal Region) (ABIN374864)

Target: AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser AOC3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-AOC3 Antikörper

Bindungsspezifität: C-Term, Internal Region

Sequenz: C-DEDPSFYSAD SIY

Spezifität: This antibody detects internal AOC3 / VAP1.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, RatSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region (near the C Terminus) of the protein sequence according to NP_003725.1 Genename: AOC3

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: Preliminary experiments gave an approx 55 kDa band in Human Adipose andLung lysates after 0.01 μg/mL antibody staining. Please note that currently we cannot findan explanation in the literature for the band we observe given the calculated size of84.5 kDa according to NP_003725.1. The 55 kDa band was successfully blocked byincubation with the immunizing peptide. We would appreciate any feedback from peoplein the field - have any results been reported with other antibodies/lysates? Have anyfurther splice variants/modified forms been reported?Immunohistochemistry on paraffin sections: 3-5 μg/mL. In Human Liver shows membranousstaining in sinusoid lining cells surrounding hepatocytes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Details zu AOC3

Target: AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))

Andere Bezeichnung: AOC3 / VAP1

Hintergrund: VAP1, upregulated in response to inflammation, is a cell adhesion protein that participates in lymphocyte recirculation, by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells, in an L selectin independent fashion. VAP1 also displays a monoamine oxidase activity, catalyzing the deamination of methylamine to produce formaldehyde. Upregulation of VAP1 has been reported in Alzheimer's disease, where increased levels of formaldehyde are responsible for b amyloid cross linkage and plaque formation, significantly contributing to the pathogenesis of this disease.Synonyms: Copper amine oxidase, HPAO, Membrane primary amine oxidase, Semicarbazide-sensitive amine oxidase, Vascular adhesion protein 1

Gen-ID: 8639, 9606

UniProt: Q16853

Pathways: Feeding Behaviour