POMC Antikörper (C-Term)

Produktnummer ABIN374549

Der Ziege Polyklonal anti-POMC Antikörper wird verwendet zum Nachweis von POMC in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC (p).

Kurzübersicht für POMC Antikörper (C-Term) (ABIN374549)

Target: POMC (Proopiomelanocortin (POMC))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser POMC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-POMC Antikörper

Bindungsspezifität: C-Term

Sequenz: C-NAIIKNAYKK GE

Spezifität: In Western blot, this antibody appears to react with the full length precursor protein only.

Kreuzreaktivität (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Human and Mouse.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Synthetic Peptide corresponding to C-Terminal of Human POMC (according to NP_000930.1, NP_001030333.1) Genename: POMC

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit Dilution: 1/32,000. Western Blot: Use at 0.01-0.03 μg/mL. Detects a band of approx. 26 kDa band in lysates of Mouse pituitary cell line NIH3T3. Immunohistochemistry: Use at 2-4 μg/mL. In Paraffin Embedded Human Adrenal Gland shows strong cytoplasm staining in selectcells in the medulla.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month (in aliquots) at -20 °C for longer.

Details zu POMC

Target: POMC (Proopiomelanocortin (POMC))

Andere Bezeichnung: POMC

Hintergrund: POMC is a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used, adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. Mutations in POMC gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.Synonyms: Corticotropin-Lipotropin, Pro-opiomelanocortin

Molekulargewicht: 29.4kDa (Human NP_000930.1) and 26.7kDa (Mouse NP_032921.1).

Gen-ID: 5443, 9606

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour