ALMS1 Antikörper (C-Term)

Produktnummer ABIN374311

Produktdetails anti-ALMS1 Antikörper

Target: ALMS1 (Alstrom Syndrome 1 (ALMS1))

Bindungsspezifität: C-Term

Reaktivität: Human, Ratte, Rind (Kuh), Hund, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ALMS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequenz: C-RVTNQLLGRK VPWD

Spezifität: This antibody detects ALMS1 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_055935.4 Genename: ALMS1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: Preliminary experiments gave bands at approx 200 kDa and 70 kDa in lysatesof Human T cell line Jurkat after 0.3 μg/mL antibody staining. Please note that currently wecannot find an explanation in the literature for the bands we observe given the calculatedsize of 460 kDa according to NP_055935.3. Both detected bands were successfully blockedby incubation with the immunizing peptide (and BLAST results with the immunizingpeptide sequence did not identify any other proteins to explain the additional bands). Wewould appreciate any feedback from people in the field - have any results been reportedwith other antibodies/lysates? Have any further splice variants/modified forms beenreported?Immunohistochemistry: 1-3 μg/mL. In paraffin embedded Human Testis shows staining ofcytoplasm in spermatocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu ALMS1

Target: ALMS1 (Alstrom Syndrome 1 (ALMS1))

Andere Bezeichnung: ALMS1 (ALMS1 Produkte)

Synonyme: ALMS1 antikoerper, ALSS antikoerper, bbb antikoerper, ALMS1, centrosome and basal body associated protein antikoerper, Alstrom syndrome 1 antikoerper, putative ALMS1-like protein antikoerper, ALMS1, centrosome and basal body associated antikoerper, ALMS1 antikoerper, alms1 antikoerper, LOC459325 antikoerper, Alms1 antikoerper

Hintergrund: ALMS1 contains a large tandem-repeat domain that contains no cysteine residues. Mutations in this gene have been associated with Alstrom syndrome. The encoded protein may play a role in hearing, sight, obesity, and liver function. Alternative splice variants have been described but their full length sequences have not been determined.Synonyms: Alstrom syndrome protein 1, KIAA0328

Gen-ID: 7840

NCBI Accession: NP_055935

UniProt: Q8TCU4

Pathways: Sensory Perception of Sound, Carbohydrate Homeostasis, M Phase