CDT1 Antikörper (C-Term)

Produktnummer ABIN374262

Der Ziege Polyklonal anti-CDT1 Antikörper (ABIN374262) detektiert spezifisch CDT1 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CDT1 Antikörper (C-Term) (ABIN374262)

Target: CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CDT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-CDT1 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-ARLAHQTRAE EGL

Spezifität: This antibody detects CDT1 at C-term.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_112190.2. Genename: CDT1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 64000. Western Blot: 1-3 μg/mL. Approx 65 kDa band observed in Human Brain lysates (predictedMW of 65 kDa according to NP_112190.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu CDT1

Target: CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))

Andere Bezeichnung: CDT1

Hintergrund: Cdt1 and its inhibitor Geminin are important regulators of replication licensing. A critical balance between both proteins functions to ensure that each origin only fires once during the cell cycle. Cdt1 cooperates with Cdc6 to promote the loading of the MCM complex onto chromatin to form the pre-replication complex. Overexpression of Cdt1 leads to aberrant replication and activation of DNA damage checkpoints. It is also associated with a predisposition for malignant transformation.Synonyms: DNA replication factor Cdt1, DUB, Double parked homolog, RIS2

Gen-ID: 81620

NCBI Accession: NP_112190

UniProt: Q9H211

Pathways: MAPK Signalweg, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA