JAG1 Antikörper (AA 110-125)

Produktnummer ABIN372790

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch JAG1 in WB, IF, IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte und Schimpanse.

Kurzübersicht für JAG1 Antikörper (AA 110-125) (ABIN372790)

Target: JAG1 (Jagged 1 (JAG1))

Reaktivität: Human, Maus, Ratte, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JAG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-JAG1 Antikörper

Bindungsspezifität: AA 110-125

Spezifität: Recognizes Human Jagged-1 (JAG1).

Kreuzreaktivität (Details): Species reactivity (expected):Chimpanzee, Rat and Mouse (100 % homology).Canine (81 %) and Xenopus laevis (85 %).
Species reactivity (tested):Human.

Aufreinigung: Protein A Chromatography.

Immunogen: Synthetic peptide corresponding to aa 110-125 of Human Jagged-1 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/2500-1/10000. Western Blot: 1/250-1/1000. Detects a band approximately 150 kD in size corresponding toJagged-1 in Mouse liver whole cell lysate and a 70 kD band in Human brain and kidneylysates. Immunocytochemistry. Immunofluorescence: 1/100-1/500. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.53 mg/mL

Buffer: PBS, pH 7.2 containing 0.01 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu JAG1

Target: JAG1 (Jagged 1 (JAG1))

Andere Bezeichnung: CD339 / JAG1

Hintergrund: Jagged-1 (JAG1) is a cell surface protein which is one of several ligands that activate Notch and related receptors. JAG1 functions in an embryologically important signaling pathway and has also been shown to play a role in hematopoiesis. JAG1 inhibits proliferation of cd34+ macrophage progenitor cells and a JAG1 gene abnormality may be an aggravating factor in extrahepatic biliary atresia. Activation of NOTCH1 signaling by JAG1 induces monocyte-derived dendritic cell maturation in vitro. JAG1 interaction with NOTCH1 on tumor cells dramatically induces proliferation and inhibition of apoptosis in vitro. Either haploinsufficiency for wild-type JAG1 and/or dominant negative effects produced by mutated JAG1 are responsible for the Alagille syndrome.Synonyms: JAGL1, Jagged-1, hJ1

Gen-ID: 182

NCBI Accession: NP_000205

UniProt: P78504

Pathways: Notch Signalweg, Stem Cell Maintenance