SLC5A1 Antikörper

Produktnummer ABIN364451

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-SLC5A1 Antikörper wird verwendet zum Nachweis von SLC5A1 in Proben von Human. Er wurde validiert für WB, IHC und IHC (p).

Kurzübersicht für SLC5A1 Antikörper (ABIN364451)

Target: SLC5A1 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC5A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SLC5A1 Antikörper

Spezifität: The antibody detects 73 kD SGLT-1 from human samples and in a lesser extent from mouse and rat samples. A ~50 kD cleavage fragment can also be detected in Jurkat cell lysate.

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide surrounding amino acid 594 of human SGLT-1.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 30 % glycerol, 0.5 % BSA, 0.01 % Thimerosal.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Referenzen für anti-SLC5A1 Antikörper (ABIN364451)

Wanes, Naim, Dengler: "Proliferation and Differentiation of Intestinal Caco-2 Cells Are Maintained in Culture with Human Platelet Lysate Instead of Fetal Calf Serum." in: Cells, Vol. 10, Issue 11, (2021) (PubMed).

Details zu SLC5A1

Target: SLC5A1 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1))

Andere Bezeichnung: SLC5A1 / SGLT1

Hintergrund: Name/Gene ID: SLC5A1
Subfamily: Solute:sodium symporter
Family: Transporter

Synonyms: SLC5A1, D22S675, HSGLT1, Na(+)/glucose cotransporter 1, Na+/glucose cotransporter 1, SGLT1, NAGT, Sodium-glucose transporter 1, Sodium/glucose cotransporter 1

Gen-ID: 6523

UniProt: P13866