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Maus anti-Human IgE (Fc Region) Antikörper

ELISA, WB, FACS, Func Monoclonal Le27-NBS01 IgG1 kappa unconjugated
Produktnummer ABIN363695
  • Target Alle IgE Produkte
    IgE
    Bindungsspezifität
    • 30
    • 19
    • 17
    • 1
    • 1
    Fc Region
    Reaktivität
    • 51
    • 31
    • 10
    • 10
    • 5
    • 4
    • 2
    • 1
    Human
    Wirt
    • 58
    • 21
    • 11
    • 10
    • 4
    • 1
    Maus
    Klonalität
    • 66
    • 32
    Monoklonal
    Applikation
    • 55
    • 40
    • 30
    • 22
    • 20
    • 15
    • 12
    • 10
    • 10
    • 8
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
    Spezifität
    Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes
    Produktmerkmale
    IGHE, chromosome 14q32.33
    Aufreinigung
    Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
    Reinheit
    > 95 %, tested by analytical Gel Permeation Chromatography
    Sterilität
    Sterile filtered
    Immunogen
    purified human myeloma IgE
    Klon
    Le27-NBS01
    Isotyp
    IgG1 kappa
  • Applikationshinweise
    For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions less than 1:100 Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    PBS, pH=7.2, sterile filtered, contains no NaN3
    Konservierungsmittel
    Azide free
    Handhabung
    Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
    Lagerung
    4 °C
  • Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).

  • Target
    IgE
    Abstract
    IgE Produkte
    Hintergrund
    The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.
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