GRP78 Antikörper (Internal Region)

Produktnummer ABIN363671

Der Kaninchen Polyklonal anti-GRP78 Antikörper wird verwendet zum Nachweis von GRP78 in Proben von Human, Maus, Ratte, Drosophila melanogaster, Schaf und Huhn. Er wurde validiert für WB, ICC, IF und FACS.

Kurzübersicht für GRP78 Antikörper (Internal Region) (ABIN363671)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reaktivität: Human, Maus, Ratte, Drosophila melanogaster, Schaf, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRP78 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-GRP78 Antikörper

Bindungsspezifität: AA 250-300, Internal Region

Spezifität: Reacts with GRP-78 protein from human, mouse, rat, Drosophila, sheep and chicken

Kreuzreaktivität (Details): Predicted to cross reacts with proteins from Bovine, C elegans, Primate, Zebrafish due to 100 % sequence homology.

Aufreinigung: Antigen affinity purified

Immunogen: Synthetic peptide derived from internal domain of the human Grp78 protein. Within residues 250-300.

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
WB(0.5 μg/mL) FC(1:50) ICC/ IF(1:50)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.67 mg/mL

Buffer: PBS, glycerol 30 % , Sodium azide 0.1 %

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Hu, Mu, Rt, Ch, Dr, Sh, Bv, Ce, Pm, Ze ovine (100 % ), Zebrafish (100 %), Primate (100 %), C. elegans (100 %).

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Haltbarkeit: 6 months

Details zu GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Andere Bezeichnung: 78 kDa Glucose-Regulated Protein (GRP-78)

Hintergrund: Categorized as stress response proteins, BiPs play an important role in folding and assembly of nascent proteins and in scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5- nontranslated region of BiP mRNA. BiP IRES activity increases when cells are heat-stressed. GRP78 is also important in maintenance of cell homeostasis and the prevention of apoptosis. Levels of GRP78 protein are reduced in brains of Alzheimer's disease patients.

Gen-ID: 3309

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling