SMC1A Antikörper (AA 955-959)

Produktnummer ABIN362823

Der Kaninchen Polyklonal anti-SMC1A Antikörper (ABIN362823) detektiert spezifisch SMC1A in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für SMC1A Antikörper (AA 955-959) (ABIN362823)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMC1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-SMC1A Antikörper

Bindungsspezifität: AA 955-959

Spezifität: The antibody detects endogenous level of total SMC1 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around AA 955-959 (G-S-S-Q-G) derived from Human SMC1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Details zu SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Andere Bezeichnung: SMC1

Hintergrund: Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Molekulargewicht: 145 kDa

NCBI Accession: NP_006297

UniProt: Q14683

Pathways: Stem Cell Maintenance