TRKB Antikörper (pTyr515)

Produktnummer ABIN362423

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-TRKB Antikörper wird verwendet zum Nachweis von TRKB in Proben von Human, Ratte und Maus. Er wurde validiert für IF.

Kurzübersicht für TRKB Antikörper (pTyr515) (ABIN362423)

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRKB Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF)

Produktdetails anti-TRKB Antikörper

Bindungsspezifität: pTyr515

Spezifität: The antibody detects endogenous level of TrkB only when phosphorylated at tyrosine 515.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr515 (P-Q-Y (p) -F-G) derived from Human TrkB. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-TRKB Antikörper (ABIN362423)

Wu, Hill, Klug, van den Buuse: "Sex-specific and region-specific changes in BDNF-TrkB signalling in the hippocampus of 5-HT1A receptor and BDNF single and double mutant mice." in: Brain research, Vol. 1452, pp. 10-7, (2012) (PubMed).

Li, Liu: "The novel squamosamide derivative FLZ enhances BDNF/TrkB/CREB signaling and inhibits neuronal apoptosis in APP/PS1 mice." in: Acta pharmacologica Sinica, Vol. 31, Issue 3, pp. 265-72, (2010) (PubMed).

Details zu TRKB

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Andere Bezeichnung: TrkB

Hintergrund: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.

Molekulargewicht: 140 kDa

NCBI Accession: NP_001007

UniProt: Q16620

Pathways: RTK Signalweg, Neurotrophin Signalübertragung, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport