Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-JunB Antikörper wird verwendet zum Nachweis von JunB in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC.
The antibody detects endogenous level of JunB only when phosphorylated at serine 259.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer259 (P-V-S (p) -P-I) derived from Human JunB. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Vartanian, Masri, Martin, Cloninger, Holmes, Artinian, Funk, Ruegg, Gera: "AP-1 regulates cyclin D1 and c-MYC transcription in an AKT-dependent manner in response to mTOR inhibition: role of AIP4/Itch-mediated JUNB degradation." in: Molecular cancer research : MCR, Vol. 9, Issue 1, pp. 115-30, (2011) (PubMed).
Target
JunB (JUNB)
(Jun B Proto-Oncogene (JUNB))
Andere Bezeichnung
JunB
Hintergrund
Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.