MEK 1,2 (Phospho-Ser218,222 ) Antikörper

Antigen: MEK 1,2 (Phospho-Ser218,222 )

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus

Applikation: Western Blot (WB)

Produktnummer: ABIN361507

Produktbeschreibung

Format: Affinity-purified

Beschreibung: MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993, Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991, Seger et al., 1991, Crews et al., 1992). Anti-Phospho Ser218/222 MEK 1/2 Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45k MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95k was also observed.

Spezifität: Specific for the ~45k MEK 1/2 protein phosphorylated at Ser218/222 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Anwendungen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Reinigung: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Lagerung: Store at -20 °C, stable for 1 year

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45k MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95k was also observed.