Tryptophan Hydroxylase 1 (TPH1) (pSer260) Antikörper

Antigen: Tryptophan Hydroxylase 1 (TPH1)

Synonyme: tph, tph1, tphD1, SERGEF, TPH1, TPH2, TPRH, TRPH, MGC119994, Tph, TPH, LOC100229598

Epitope: pSer260

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Ratte (Rattus), Human

Applikation: Western Blot (WB)

Produktnummer: ABIN361499

Produktbeschreibung

Weitere Bezeichnung: Phospho-Ser260 Tryptophan Hydroxylase

Format: Affinity-purified

Beschreibung: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000). Anti-Phospho Ser260 Tryptophan Hydroxylase Western blot of rat brainstem lysate showing specific immunolabeling of the ~55k TPH protein phosphorylated at Ser260. The labeling is specifically blocked by the phosphopeptide (Phos-pep) used as antigen. The corresponding non-phosphopeptide (Nonphos-pep) did not block the immunolabeling.

Spezifität: Specific for the ~55k tryptophan hydroxylase protein phosphorylated at Ser260.

Anwendungen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Reinigung: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Lagerung: Store at -20 °C, stable for 1 year

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blots of rat brainstem lysate showing specific immunolabeling of the ~55k TPH protein phosphorylated at Ser260. The labeling is specifically blocked by the phosphopeptide (Phos-pep) used as antigen. The corresponding non-phosphopeptide (Nonphos-pep) did not block the immunolabeling.