DARPP32 Antikörper (pThr75)

Produktnummer ABIN361463

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-DARPP32 Antikörper wird verwendet zum Nachweis von DARPP32 in Proben von Ratte. Er wurde validiert für WB und ELISA.

Kurzübersicht für DARPP32 Antikörper (pThr75) (ABIN361463)

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DARPP32 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-DARPP32 Antikörper

Bindungsspezifität: pThr75

Spezifität: Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr75. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Kreuzreaktivität: Maus, Ratte

Homologie: bovine, canine, chicken, human, non-human primates

Produktmerkmale: Our Anti-DARPP-32 (Thr75) Antibody phosphospecific primary antibody is rabbit polyclonal. It detects DARPP-32 (Thr75) and is antigen affinity purified. It is great for use in ELISA, WB.

Aufreinigung: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 of rat DARPP-32 conjugated to keyhole limpet hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-DARPP32 Antikörper (ABIN361463)

Gomez, Midde, Mactutus, Booze, Zhu: "Environmental enrichment alters nicotine-mediated locomotor sensitization and phosphorylation of DARPP-32 and CREB in rat prefrontal cortex." in: PLoS ONE, Vol. 7, Issue 8, pp. e44149, (2013) (PubMed).

Cantrup, Sathanantham, Rushlow, Rajakumar: "Chronic hyperdopaminergic activity of schizophrenia is associated with increased ΔFosB levels and cdk-5 signaling in the nucleus accumbens." in: Neuroscience, Vol. 222, pp. 124-35, (2012) (PubMed).

Napolitano, Bonito-Oliva, Federici, Carta, Errico, Magara, Martella, Nistica: "Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 33, pp. 11043-56, (2010) (PubMed).

Details zu DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Andere Bezeichnung: PPP1R1B

Hintergrund:

Synonyms: DARPP32 antibody, DARPP-32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody, Dopamine and cAMP regulated phosphoprotein DARPP32 antibody, Dopamine- and cAMP-regulated neuronal phosphoprotein antibody, FLJ20940 antibody, IPPD antibody, Neuronal phosphoprotein DARPP 32 antibody, PPP1R1B antibody, PPR1B_HUMAN antibody, Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody, Protein phosphatase 1 regulatory inhibitor subunit 1B antibody, Protein phosphatase 1 regulatory subunit 1B antibody

Description Logic: Our Anti-DARPP-32 (Thr75) Antibody phosphospecific primary antibody is rabbit polyclonal. It detects DARPP-32 (Thr75) and is antigen affinity purified. It is great for use in ELISA, WB.

Gene Name Alternatives: PPP1R1B

Molekulargewicht: 32 kDa

Gen-ID: 360616

UniProt: Q6J4I0