DARPP32 Antikörper (pThr34)

Produktnummer ABIN361462

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch DARPP32 in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Ratte. Es wurde in 8+ Publikationen zitiert.

Kurzübersicht für DARPP32 Antikörper (pThr34) (ABIN361462)

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DARPP32 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-DARPP32 Antikörper

Bindungsspezifität: pThr34

Spezifität: Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr34. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Kreuzreaktivität: Maus, Ratte

Homologie: bovine, canine, chicken, human, non-human primates

Produktmerkmale: Our DARPP-32 (Thr34) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects DARPP-32 (Thr34) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Aufreinigung: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-DARPP32 Antikörper (ABIN361462)

DelGuidice, Latapy, Rampino, Khlghatyan, Lemasson, Gelao, Quarto, Rizzo, Barbeau, Lamarre, Bertolino, Blasi, Beaulieu: "FXR1P is a GSK3β substrate regulating mood and emotion processing." in: Proceedings of the National Academy of Sciences of the United States of America, (2015) (PubMed).

Gomez, Midde, Mactutus, Booze, Zhu: "Environmental enrichment alters nicotine-mediated locomotor sensitization and phosphorylation of DARPP-32 and CREB in rat prefrontal cortex." in: PLoS ONE, Vol. 7, Issue 8, pp. e44149, (2013) (PubMed).

Marazziti, Di Pietro, Mandillo, Golini, Matteoni, Tocchini-Valentini: "Absence of the GPR37/PAEL receptor impairs striatal Akt and ERK2 phosphorylation, DeltaFosB expression, and conditioned place preference to amphetamine and cocaine." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 6, pp. 2071-81, (2011) (PubMed).

Daigle, Wetsel, Caron: "Opposite function of dopamine D1 and N-methyl-D-aspartate receptors in striatal cannabinoid-mediated signaling." in: The European journal of neuroscience, Vol. 34, Issue 9, pp. 1378-89, (2011) (PubMed).

Napolitano, Bonito-Oliva, Federici, Carta, Errico, Magara, Martella, Nistica: "Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 33, pp. 11043-56, (2010) (PubMed).

Santini, Sgambato-Faure, Li, Savasta, Dovero, Fisone, Bezard: "Distinct changes in cAMP and extracellular signal-regulated protein kinase signalling in L-DOPA-induced dyskinesia." in: PLoS ONE, Vol. 5, Issue 8, pp. e12322, (2010) (PubMed).

Niculescu, Perrine, Miller, Ehrlich, Unterwald: "Trk: a neuromodulator of age-specific behavioral and neurochemical responses to cocaine in mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 5, pp. 1198-207, (2008) (PubMed).

Beaulieu, Sotnikova, Gainetdinov, Caron: "Paradoxical striatal cellular signaling responses to psychostimulants in hyperactive mice." in: The Journal of biological chemistry, Vol. 281, Issue 43, pp. 32072-80, (2006) (PubMed).

Details zu DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Andere Bezeichnung: PPP1R1B

Hintergrund:

Synonyms: DARPP32 antibody, DARPP-32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody, Dopamine and cAMP regulated phosphoprotein DARPP32 antibody, Dopamine- and cAMP-regulated neuronal phosphoprotein antibody, FLJ20940 antibody, IPPD antibody, Neuronal phosphoprotein DARPP 32 antibody, PPP1R1B antibody, PPR1B_HUMAN antibody, Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody, Protein phosphatase 1 regulatory inhibitor subunit 1B antibody, Protein phosphatase 1 regulatory subunit 1B antibody

Description Logic: Our DARPP-32 (Thr34) rabbit polyclonal phosphospecific primary antibody is produced . It detects DARPP-32 (Thr34) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: PPP1R1B

Molekulargewicht: 32 kDa

Gen-ID: 360616

UniProt: Q6J4I0