NMDA Receptor, NR2A Subunit (0) Antikörper

Antigen: NMDA Receptor, NR2A Subunit (0)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunpräzipitation (IP)

Produktnummer: ABIN361396

Produktbeschreibung

Weitere Bezeichnung: NMDA Receptor, NR2A Subunit

Format: Affinity-purified

Beschreibung: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Anti-NMDA Receptor, NR2A Subunit Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDA receptor.

Spezifität: Specific for the ~180k NR2A subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2A subunit of NMDAR. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.

Anwendungen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:1000 to 1:2000 IP: 3 µl per 200 µg lysate Quality Control: Western blots performed on each lot.

Reinigung: Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Lagerung: Store at -20 °C, stable for 1 year

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blots of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDA receptor.