HMGB1 Antikörper (N-Term)

Produktnummer ABIN356227

Der Maus Monoklonal anti-HMGB1 Antikörper (Klon J2E1) (ABIN356227) detektiert spezifisch HMGB1 in WB, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für HMGB1 Antikörper (N-Term) (ABIN356227)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HMGB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: J2E1

Produktdetails anti-HMGB1 Antikörper

Bindungsspezifität: AA 1-215, N-Term

Spezifität: The antibody recognizes human HMGB1. Other species not tested.

Produktmerkmale: Synonyms: High mobility group protein 1, HMG1, HMG-1, High mobility group protein B1, HMGB-1,Amphoterin

Aufreinigung: Protein-G affinity chromatography

Immunogen: Recombinant human HMGB1 (1-215aa) purified from High Five (Trichoplusia ni) insect cells.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot (1: 500 - 1: 2,000), recommended starting dilution is 1: 1,000. Immunohistochemistry (1: 100 - 1: 300), recommended starting dilution is 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: HMGB1

Hintergrund: High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor.Synonyms: Amphoterin, HMG-1, HMG1, HMGB-1, High mobility group protein 1, High mobility group protein B1

Gen-ID: 3146

UniProt: P09429

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome