Retinoblastoma-Like Protein 2 (RBL2) Antikörper

Antigen: Retinoblastoma-Like Protein 2 (RBL2)

Synonyme: Rb2, P130, FLJ26459, p130

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: ELISA, Western Blot (WB), Immunhistochemie (IHC), Immunfluoreszenz (IF)

Produktnummer: ABIN349607

Produktbeschreibung

Weitere Bezeichnung: Spa310 (pRb2/p130)

Gen-ID: CAA52671

Swiss-Prot: Q08999

Immunogen: This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the Spa310 sequence of pRb2/p130 protein. A residue of cysteine was added to facilitate coupling.

Format: Liquid (sterile filtered)

Beschreibung: Spa310 is a 39 aa-long polypeptide encoded by a sequence which resides in the spacer region of the tumor suppressor Rb2 gene. Rb2 is a member of the retinoblastoma (Rb) gene family. Proteins in this family, which also include pRb/p105 and pRb/p107, are important cellular factors which play well-recognized roles as tumor and growth suppressors. Both p107 and pRb2/p130 share the ability to physically interact and inhibit the kinase activity of the Cdk2/Cyclin A and Cdk2/Cyclin E complexes, which play critical roles in cell cycle regulation. Spa310 is the region of the pRb2/p130 protein that is responsible for Cdk2/Cyclin E/A inhibition. Spa310 has also been shown to suppress cell growth as observed by colony formation, and to reduce volume of tumor growth in nude mice, likely through arrest in the G 0 /G 1 phase of the cell cycle. Understandably, the Spa310 small molecule represents a potentially significant pharmaceutical product in the treatment of hyperproliferative disorders.

Spezifität: This antiserum is directed against Spa310 and reacts with the Spa3120 domain of pRb2/p130 from human tissues. Based on the sequence we expect this antibody to react as well Spa310 from chimpanzee and orangutan, and with lesser affinity, Spa310 from horse, dog, bovine, rat and opossum.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:75,000 to 1:100,000 WESTERN BLOT 1:500 to 1:5,000 IMMUNOHISTOCHEMISTRY User Optimized IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to pRb2/p130, and a band approximately 4.2kDa in size corresponding to Spa310 peptide (latter not shown), by western blotting in the appropriate cell lysate or extract.

Konzentration: 1.39 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.1% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20o C prior to opening. Aliquot contents and freeze at -20o C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4o C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot using affinity purified anti-Spa310 antibody shows detection of endogenous pRb2/p130 protein in whole LNCaP cell extracts. The band at ~130 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of pRb2/p130. The membrane was blocked overnight with a milk buffer at 4o C. Primary antibody was diluted 1:500 and reacted with the membrane overnight at 4o C. ECL was used for detection. Personal communication, Ang Sun, Sbarro Institute for Cancer Research and Molecular Medicine, Temple University, Philadelphia, PA.