Heat Shock Protein Hsp90 beta (hsp90b) Antikörper

Antigen: Heat Shock Protein Hsp90 beta (hsp90b)

Klonalität: Monoklonal (MBH90B)

Wirt: Maus

Reaktivität: Human, Maus, Rind (Kuh)

Applikation: Immunpräzipitation (IP), Western Blot (WB), Immunzytochemie (ICC)

Produktnummer: ABIN343707

Produktbeschreibung

Weitere Bezeichnung: Hsp90 beta

Immunogen: Peptide corresponding to the EEVHHGEEEVEC sequence within N-terminal part of humanHsp90.

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: MBH90B

Beschreibung: Hsp90 beta (heat shock protein 90 beta) is a constitutively expressed isoform of Hsp90, oneof the most abundant chaperones in the cytosol of eukaryotic cells. Hsp90 interacts withvarious proteins, including protein kinases and transcription factors, and either facilitatestheir stabilization and activation or directs them for proteasomal degradation. Hsp90 thusaffects multiple signaling pathways and biological processes and modulation of this singletarget offers the prospect of simultaneous intervence to various key points of oncogenictransformation. Hsp90 operates as a dimer in a conformational cycle driven by ATP bindingand hydrolysis.

Spezifität: The antibody MBH90B recognizes the EEVHHG epitope within the N-terminal part of Hsp90beta an ubiquitously expressed protein with calculated Mw of 83.3 kDa, however, migratingas a 90 kDa band under reducing SDS-PAGE conditions.

Anwendungen

Applikationshinweise: Immunoprecipitation - Recommended dilution: 1 µg/ml. Western Blotting - Recommended dilution: 1 µg/ml. Immunocytochemistry - Recommended dilution: 1 µg/ml

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by protein-A affinity chromatography.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Signaltransduktion, Hitzeschockproteine, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blotting analysis of MBH90B reactivity with various cell lines and recombinant Hsp90Alpha and Hsp90Beta protein.