14-3-3 Binding Motif (pSer) Antikörper

Antigen: 14-3-3 Binding Motif

Epitope: pSer

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Western Blot (WB), Immunpräzipitation (IP), ELISA, Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN337475

Produktbeschreibung

Weitere Bezeichnung: 14-3-3 Binding Motif, pSer

Immunogen: Synthetic peptide, Synthetic phospho- (Ser) 14-3-3-binding-motif peptides.

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Gene: 14-3-3 Binding Motif

Spezifität: Binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position. Antibody binding is phospho-specific and largely independent of other surrounding amino acids. Weakly cross-reacts with sequences containing phospho-Thr instead of phospho-Ser in this motif, with sequences containing phospho-Ser surrounded by Phe at the +1 position, with Arg/Lys at the -3 position. No cross-reactivity is observed with corresponding non-phosphorylated sequences or with other phospho- Thr/Ser/Tyr -containing motifs.

Anwendungen

Applikationshinweise: WB (1:1000), IP (1:50), ELISA (1:1000), IHC-P

Reinigung: Immunoaffinity Chromatography

Buffer: 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol.

Beschränkungen: Nur für Forschungszwecke einsetzbar