SMAD9 Antikörper (AA 166-179)

Produktnummer ABIN337365

Der Ziege Polyklonal anti-SMAD9 Antikörper (ABIN337365) detektiert spezifisch SMAD9 in WB, ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Hund, Fledermaus, Huhn, Rind (Kuh), Hamster, Pferd, Affe, Schwein und Kaninchen.

Kurzübersicht für SMAD9 Antikörper (AA 166-179) (ABIN337365)

Target: SMAD9 (SMAD Family Member 9 (SMAD9))

Reaktivität: Human, Maus, Ratte, Hund, Fledermaus, Huhn, Rind (Kuh), Hamster, Pferd, Affe, Schwein, Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SMAD9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SMAD9 Antikörper

Bindungsspezifität: AA 166-179

Spezifität: Human SMAD9. This antibody is not expected to cross-react with SMAD1 and SMAD5.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Turkey, Chicken (100%) Opossum, Platypus, Lizard (93%) Stickleback (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-HSEPLMPHNATYPD from an internal region of human SMAD9 (NP_005896.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Turkey, Chicken (100%), Opossum, Platypus, Lizard (93%), Stickleback (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu SMAD9

Target: SMAD9 (SMAD Family Member 9 (SMAD9))

Andere Bezeichnung: SMAD9

Hintergrund: Name/Gene ID: SMAD9

Synonyms: SMAD9, MAD homolog 9, SMAD 9, SMAD8A, SMAD8, Smad9, MADH9, SMAD8B, MADH6, Mothers against DPP homolog 9, SMAD family member 9

Gen-ID: 4093

NCBI Accession: NP_005896

UniProt: O15198