PPP2R3A Antikörper (AA 1073-1085)

Produktnummer ABIN337311

Der Ziege Polyklonal anti-PPP2R3A Antikörper (ABIN337311) detektiert spezifisch PPP2R3A in WB, ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Pferd, Kaninchen, Schwein und Affe.

Kurzübersicht für PPP2R3A Antikörper (AA 1073-1085) (ABIN337311)

Target: PPP2R3A (Protein Phosphatase 2, Regulatory Subunit B'', alpha (PPP2R3A))

Reaktivität: Human, Maus, Pferd, Kaninchen, Schwein, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PPP2R3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PPP2R3A Antikörper

Bindungsspezifität: AA 1073-1085

Spezifität: Human PPP2R3A. This antibody is expected to recognise both reported isoforms (NP_002709.2, NP_871626.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Horse, Rabbit, Pig, Lizard (100%) Rat, Hamster, Bat, Bovine, Opossum, Turkey, Chicken (92%) Dog (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-QKDVENDGPEPSD from an internal region of human PPP2R3A (NP_002709.2, NP_871626.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Horse, Rabbit, Pig, Lizard (100%), Rat, Hamster, Bat, Bovine, Opossum, Turkey, Chicken (92%), Dog (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:16000), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PPP2R3A

Target: PPP2R3A (Protein Phosphatase 2, Regulatory Subunit B'', alpha (PPP2R3A))

Andere Bezeichnung: PPP2R3A / PR130

Hintergrund: Name/Gene ID: PPP2R3A

Synonyms: PPP2R3A, PR130, PR72, PPP2R3, PP2A, subunit B, R3 isoform

Gen-ID: 5523

NCBI Accession: NP_002709, NP_871626

UniProt: Q06190

Pathways: PI3K-Akt Signalweg